Can widely used cell type markers predict the suitability of immortalized or primary mammary epithelial cell models?

Biological research: BR

Xiao Huang¹  thi¹  Edgar Corneille Ontsouka¹  Michael Lü¹  Janique Sabina Bertschi²  Stefan Mü² 

[1] Faculty of Medicine, Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland;Swiss National Center of Competence in Research, NCCR TransCure, University of Bern, Bern, Switzerland

关键词: Cell characterization;    Flow cytometry;    Gene expression;    Lactation marker;    Mammalian;    Mammary epithelial cell;   

DOI  :  10.1186/s40659-015-0063-2

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Mammary cell cultures are convenient tools for in vitro studies of mammary gland biology. However, the heterogeneity of mammary cell types, e.g., glandular milk secretory epithelial or myoepithelial cells, often complicates the interpretation of cell-based data. The present study was undertaken to determine the relevance of bovine primary mammary epithelial cells isolated from American Holstein (bMECUS) or Swiss Holstein–Friesian (bMECCH) cows, and of primary bovine mammary alveolar epithelial cells stably transfected with simian virus-40 (SV-40) large T-antigen (MAC-T) for in vitro analyses. This was evaluated by testing their expression pattern of cytokeratin (CK) 7, 18, 19, vimentin, and α-smooth muscle actin (α-SMA). The expression of the listed markers was assessed using real-time quantitative PCR, flow cytometry and immunofluorescence microscopy. Characteristic markers of the mesenchymal (vimentin), myoepithelial (α-SMA) and glandular secretory cells (CKs) showed differential expression among the studied cell cultures, partly depending on the analytical method used. The relative mRNA expression of vimentin, CK7 and CK19, respectively, was lower (P 

【 授权许可】

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