Evaluation of methylation status in glutation S-transferase P1(GSTP1) gene promoter in human breast cancer and its relation to tumor grade and stage

Scientific Research and Essays

Shohreh Alizadeh Shargh¹ 

关键词:  ;    GSTP1;    promoter;    methylation;    pattern;    breast cancer.;   

DOI  :  

学科分类：社会科学、人文和艺术（综合）

来源: Academic Journals

【 摘 要 】

Glutation S-transferase P1 (GSTP1) gene methylation in promoter CpG islands has been described as a specific biomarker for many types of cancer including breast cancer as a tumor suppressor gene. At the present study we found that the GSTP1 gene promoter to be methylated in breast cancer tissues. For studying the difference of sequence in hypermethylated GSTP1 promoter in cancer tissues and non methylated status in normal tissues, we analyzed the cytosine methylation status as epigenetic changes in 50 tumors from patients with breast cancer and 50 normal breast tissues that were obtained from the adjacent regions of the breast tumors of the same patients. In order to study the promoter methylation status for GSTP1 gene in breast cancer, 40 CpG sites [nucleotide(nt) 197, 190, 187, 185, 183, 182, 176, 162, 155, 152, 148, 145, 141, 132, 127, 124, 112, 109, 101, 99, 81, 77, 74, 71, 54, 53, 48, 47, 43, 42, 40, 38, 23, 22, 15, 14, 13, 11, 8, 4] were screened. The GSTP1 methylation was detected in 41.3% of the breast tumors which was associated with higher tumor grade (p=0.467) and tumor stage (p = 0.048).

【 授权许可】

CC BY