Isolation of Hepatic Progenitor Cells from Human Liver with Cirrhosis Secondary to Biliary Atresia Using EpCAM or Thy-1 Markers

Cell Medicine

Takayoshi Tokiwa¹  Taisuke Yamazaki¹  Atsuko Nakazawa²  Shin Enosawa³  Takanobu Shigeta⁴  Akinari Fukuda⁴  Seisuke Sakamoto⁴  Mureo Kasahara⁴ 

[1] * Department of Liver Cell Biology, Kohno Clinical Medicine Research Institute, Shinagawa-ku, Tokyo, Japan;§ Department of Clinical Pathology, National Center for Child Health and Development, Tokyo, Japan;† Division for Advanced Medical Science, National Center for Child Health and Development, Tokyo, Japan;‡ Division of Surgery, National Center for Child Health and Development, Tokyo, Japan

关键词: Biliary atresia;    Cirrhosis;    Hepatic progenitor cells;    Flow cytometry;    EpCAM;    Thy-1;   

DOI  :  10.3727/215517912X639441

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

We sought to determine whether hepatic progenitor cells can be isolated from cirrhotic liver using epithelial cell adhesion molecule (EpCAM) or Thy-1 markers. Liver tissue with cirrhosis secondary to biliary atresia (BA) was collagenase digested, and nonparenchymal cells (NPCs) were cultivated for 24 h. Noncirrhotic NPCs derived from patients with carbamyl phosphate synthetase and ornithine transcarbamylase deficiencies were used as controls. Flow cytometric analysis demonstrated that the percentages of EpCAM- and Thy-1-positive cells were significantly higher in NPC populations derived from BA liver than in those derived from control liver. Reverse transcription polymerase chain reaction analysis revealed that EpCAM-positive sorted cells expressed EpCAM, Thy-1, albumin, and CK-19, whereas Thy-1-positive sorted cells expressed Thy-1, albumin, and CK-19. These findings indicate that EpCAM- or Thy-1-positive hepatic progenitor cells can be more efficiently isolated from BA liver than from control liver and suggest that the properties of EpCAM-positive cells are somewhat different from those of Thy-1-positive cells.

【 授权许可】

CC BY