Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats

Journal of venomous animals and toxins

Luiza Anna FV Assumpç¹  Eliane G Melo²  rgio L Lavor²  Marcus Viní²  o²  Karen M Oliveira²  Isabel R Rosado⁴  cius Gomez⁴  Carla Maria O Silva⁴  Saira MN Neves⁴  Fabí⁵  Má⁵  Guilherme R Motta⁵  Marí⁵  ola B Fukushima⁶  rio Sé⁶  Fernanda F Garcia⁶  lia M Melo⁶  ã⁷ 

[1]Departament of Agrarian and Environmental Sciences, State University of Santa Cruz, Ilhé

[2]Departamento de Clí

[3]National Institute of Sciences and Technology on Molecular Medicine, School of Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil

[4]nica e Cirurgia Veteriná

[5]ria, Escola de Veteriná

[6]ria, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

[7]us, Brazil

关键词: Conus magus;    Cone snail;    Histopathology;    Hematology;    Biochemistry;   

DOI  :  10.1186/1678-9199-20-15

学科分类：药理学

来源: BioMed Central

【 摘 要 】

Calcium channel blockers such as conotoxins have shown a great potential to reduce brain and spinal cord injury. MVIIC neuroprotective effects analyzed in in vitro models of brain and spinal cord ischemia suggest a potential role of this toxin in preventing injury after spinal cord trauma. However, previous clinical studies with MVIIC demonstrated that clinical side effects might limit the usefulness of this drug and there is no research on its systemic effects. Therefore, the present study aimed to investigate the potential toxic effects of MVIIC on organs and to evaluate clinical and blood profiles of rats submitted to spinal cord injury and treated with this marine toxin. Rats were treated with placebo or MVIIC (at doses of 15, 30, 60 or 120 pmol) intralesionally following spinal cord injury. Seven days after the toxin administration, kidney, brain, lung, heart, liver, adrenal, muscles, pancreas, spleen, stomach, and intestine were histopathologically investigated. In addition, blood samples collected from the rats were tested for any hematologic or biochemical changes. The clinical, hematologic and biochemical evaluation revealed no significant abnormalities in all groups, even in high doses. There was no significant alteration in organs, except for degenerative changes in kidneys at a dose of 120 pmol. These findings suggest that MVIIC at 15, 30 and 60 pmol are safe for intralesional administration after spinal cord injury and could be further investigated in relation to its neuroprotective effects. However, 120 pmol doses of MVIIC may provoke adverse effects on kidney tissue.

【 授权许可】

CC BY