Isolation of Genetically Diverse Marburg Viruses from Egyptian Fruit Bats

PLoS Pathogens

Marina L. Khristova¹  Pierre B. H. Formenty²  Stephen Balinandi³  Robert Downing³  Jordan W. Tappero³  Emmanuel Byaruhanga⁴  Sherif R. Zaki⁵  Christopher D. Paddock⁵  Patricia W. Greer⁵  Samuel Okware⁶  Robert Swanepoel⁷  Alan Kemp⁷  James A. Comer⁸  Jonathan S. Towner⁸  Serena A. Reeder Carroll⁸  Brian R. Amman⁸  Pierre E. Rollin⁸  David M. Miller⁸  Cesar G. Albarino⁸  James N. Mills⁸  Deborah L. Cannon⁸  Zachary D. Reed⁸  Eileen C. Farnon⁸  Thomas G. Ksiazek⁸  Stuart T. Nichol⁸  Tara K. Sealy⁸  Edward Katongole-Mbidde⁹  John T. Kayiwa⁹  Patrick Atimnedi^1,10 

[1] Biotechnology Core Facility Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America;Epidemic and Pandemic Alert and Response Department, World Health Organization, Geneva, Switzerland;Global AIDS Program, Centers for Disease Control and Prevention, Entebbe, Uganda;Ibanda District Hospital, Ibanda, Uganda;Infectious Disease Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America;Ministry of Health, Republic of Uganda, Kampala, Uganda;National Institute for Communicable Diseases, Special Pathogens Unit, Johannesburg, South Africa;Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America;Uganda Virus Research Institute, Entebbe, Uganda;Uganda Wildlife Authority, Kampala, Uganda

关键词: Bats;    Fruit bats;    Marburg virus;    Reverse transcriptase-polymerase chain reaction;    Phylogenetic analysis;    RNA viruses;    Marburg hemorrhagic fever;    RNA extraction;   

DOI  :  10.1371/journal.ppat.1000536

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

In July and September 2007, miners working in Kitaka Cave, Uganda, were diagnosed with Marburg hemorrhagic fever. The likely source of infection in the cave was Egyptian fruit bats (Rousettus aegyptiacus) based on detection of Marburg virus RNA in 31/611 (5.1%) bats, virus-specific antibody in bat sera, and isolation of genetically diverse virus from bat tissues. The virus isolates were collected nine months apart, demonstrating long-term virus circulation. The bat colony was estimated to be over 100,000 animals using mark and re-capture methods, predicting the presence of over 5,000 virus-infected bats. The genetically diverse virus genome sequences from bats and miners closely matched. These data indicate common Egyptian fruit bats can represent a major natural reservoir and source of Marburg virus with potential for spillover into humans.

【 授权许可】

CC BY