期刊论文详细信息
PLoS Pathogens
Haemophilus influenzae Infection Drives IL-17-Mediated Neutrophilic Allergic Airways Disease
Jodie L. Simpson1  Ama-Tawiah Essilfie1  Paul S. Foster1  Philip M. Hansbro1  Julie A. Preston1  Margaret L. Dunkley1  Jay C. Horvat2  Peter G. Gibson3 
[1] Centre for Asthma and Respiratory Diseases and Hunter Medical Research Institute, The University of Newcastle, Newcastle, New South Wales, Australia;Department of Respiratory and Sleep Medicine, John Hunter Hospital, New Lambton, New South Wales, Australia;Hunter Immunology, Newcastle, Australia
关键词: Neutrophils;    Respiratory infections;    Asthma;    Inflammation;    Haemophilus influenzae;    T cells;    Chlamydia infection;    Macrophages;   
DOI  :  10.1371/journal.ppat.1002244
学科分类:生物科学(综合)
来源: Public Library of Science
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【 摘 要 】

A subset of patients with stable asthma has prominent neutrophilic and reduced eosinophilic inflammation, which is associated with attenuated airways hyper-responsiveness (AHR). Haemophilus influenzae has been isolated from the airways of neutrophilic asthmatics; however, the nature of the association between infection and the development of neutrophilic asthma is not understood. Our aim was to investigate the effects of H. influenzae respiratory infection on the development of hallmark features of asthma in a mouse model of allergic airways disease (AAD). BALB/c mice were intraperitoneally sensitized to ovalbumin (OVA) and intranasally challenged with OVA 12–15 days later to induce AAD. Mice were infected with non-typeable H. influenzae during or 10 days after sensitization, and the effects of infection on the development of key features of AAD were assessed on day 16. T-helper 17 cells were enumerated by fluorescent-activated cell sorting and depleted with anti-IL-17 neutralizing antibody. We show that infection in AAD significantly reduced eosinophilic inflammation, OVA-induced IL-5, IL-13 and IFN-γ responses and AHR; however, infection increased airway neutrophil influx in response to OVA challenge. Augmented neutrophilic inflammation correlated with increased IL-17 responses and IL-17 expressing macrophages and neutrophils (early, innate) and T lymphocytes (late, adaptive) in the lung. Significantly, depletion of IL-17 completely abrogated infection-induced neutrophilic inflammation during AAD. In conclusion, H. influenzae infection synergizes with AAD to induce Th17 immune responses that drive the development of neutrophilic and suppress eosinophilic inflammation during AAD. This results in a phenotype that is similar to neutrophilic asthma. Infection-induced neutrophilic inflammation in AAD is mediated by IL-17 responses.

【 授权许可】

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