COVALENT IMMOBILIZATION OF GLUCOSE OXIDASE ON WELL-DEFINED POLYMER-SILICON WAFER HYBRIDS VIA SURFACE-INITIATED RAFT-MEDIATED PROCESS

Journal of The Chilean Chemical Society

JI, JIAYOU¹  LIU, TINGTING¹  LI, LIANG¹  XIA, KEQIANG¹ 

[1] Wuhan Institute of Technology, Wuhan, P. R. China

关键词: surface-;    initiated RAFT;    silicon;    poly(glycidyl methacrylate);    glucose oxidase.;   

DOI  :  10.4067/S0717-97072013000300017

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

A surface modification technique was developed for the functionalization of silicon surface with glucose oxidase (GOD). The silicon surface was first graft copolymerized with glycidyl methacrylate (GMA) via surface-initiated reversible addition-fragmentation chain-transfer (RAFT)-mediated process. GOD was then covalently immobilized through the ring-opening reaction between the amine groups of the GOD and the epoxide groups of the grafted GMA polymer chains. X-ray photoelectron spectroscopy (XPS) was used to characterize the surface-modified surface after each modification stage. Increasing the thickness of the polymer layer and the immobilization time could allow a great amount of GOD to be immobilized on the silicon surface. The GOD-functionalized silicon hybrids are promising candidates for the silicon-based glucose biosensors.

【 授权许可】

Unknown