Tpc1 is an important Zn(II)2Cys6 transcriptional regulator required for polarized growth and virulence in the rice blast fungus

PLoS Pathogens

Lauren S. Ryder¹  Muhammad Badaruddin¹  Darren M. Soanes¹  Ana Lilia Martinez-Rocha²  David J. Studholme²  Julio Rodríguez-Romero²  Rita Galhano³  Marie Demuez⁴  Adriana Illana⁴  Ane Sesma⁴  Nicholas J. Talbot⁴ 

[1] Centre for Plant Biotechnology and Genomics (CBGP), Universidad Politécnica de Madrid, (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Pozuelo de Alarcón, Madrid, Spain;Dept. Biotecnología y Biología Vegetal, UPM, Madrid, Spain;Disease & Stress Biology Dept. John Innes Centre, Norwich, United Kingdom;School of Biosciences, University of Exeter, Geoffrey Pope Building, Exeter, United Kingdom

关键词: Appressoria;    Autophagic cell death;    Rice blast fungus;    Transcription factors;    Fungal genetics;    Cell polarity;    DNA-binding proteins;    Gene expression;   

DOI  :  10.1371/journal.ppat.1006516

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The establishment of polarity is a critical process in pathogenic fungi, mediating infection-related morphogenesis and host tissue invasion. Here, we report the identification of TPC1 (Transcription factor for Polarity Control 1), which regulates invasive polarized growth in the rice blast fungus Magnaporthe oryzae. TPC1 encodes a putative transcription factor of the fungal Zn(II)2Cys6 family, exclusive to filamentous fungi. Tpc1-deficient mutants show severe defects in conidiogenesis, infection-associated autophagy, glycogen and lipid metabolism, and plant tissue colonisation. By tracking actin-binding proteins, septin-5 and autophagosome components, we show that Tpc1 regulates cytoskeletal dynamics and infection-associated autophagy during appressorium-mediated plant penetration. We found that Tpc1 interacts with Mst12 and modulates its DNA-binding activity, while Tpc1 nuclear localisation also depends on the MAP kinase Pmk1, consistent with the involvement of Tpc1 in this signalling pathway, which is critical for appressorium development. Importantly, Tpc1 directly regulates NOXD expression, the p22phox subunit of the fungal NADPH oxidase complex via an interaction with Mst12. Tpc1 therefore controls spatial and temporal regulation of cortical F-actin through regulation of the NADPH oxidase complex during appressorium re-polarisation. Consequently, Tpc1 is a core developmental regulator in filamentous fungi, linking the regulated synthesis of reactive oxygen species and the Pmk1 pathway, with polarity control during host invasion.

【 授权许可】

CC BY