Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies

Quimica nova

Fonseca, Maria José Vieira¹  Vicentini, Fabiana T. M. C.¹  Fonseca, Yris Maria¹  Catini, Carolina Dias¹ 

[1] Universidade de São Paulo, Ribeirão Preto, Brasil

关键词: Calendula officinalis;    narcissin;    rutin.;   

DOI  :  10.1590/S0100-40422010000600019

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A chromatographictechnique for determination of rutin and narcissin in marigold extract and topicalformulations was developed and validated. The method shows linearity over theconcentration range of 0.2 - 6.0 μg/mLof rutin (r = 0.9986) and 0.8 - 12.0 μg/mLof narcissin (r = 0.9951). The values obtained for precision and accuracy arein agreement with ICH guidelines. Both the formulation excipients and the porcineear skin samples did not interfere with the flavonoids determination. The recoveryof rutin and narcissin in skin samples added with marigold extract was 81.41%and 83.35%, respectively, which demonstrate the applicability of this methodto perform skin penetration studies.

【 授权许可】

CC BY