Analytical methodology for the determination of folates and folic acid in foods

Quimica nova

Lima-Pallone, Juliana Azevedo¹  Catharino, Rodrigo Ramos²  Godoy, Helena Teixeira² 

[1] Universidade Católica de Campinas, Campinas, Brasil;Universidade Estadual de Campinas, Campinas, Brasil

关键词: HPLC methodology;    folates;    food.;   

DOI  :  10.1590/S0100-40422006000500016

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The aim of thiswork was to develop and to validate a methodology using HPLC for the simultaneousdetermination of folates and folic acid in foods. The limits of detection andthe recovery rates for the vitamins in the certified reference materials wererespectively 5 pg/mL and 94-108% for 5-MTHF, 7 pg/mL and 97-102% for THF, 30pg/mL and 97.9-104% for 5-FTHF, 30 pg/mL and 95-107 for 10-FFA, 5 ng/mL and97-102% for FA and 5 ng/mL and 98-103% for 10-MFA. Repeatability showed a coefficientof variation below 3.9% for all the vitamins. The proposed methodology was shownto be efficient when applied to different certified reference materials, namelypig's liver (BCR487), powdered milk (BCR421) and a vegetable mixture (BCR485).

【 授权许可】

CC BY