A simple apparatus to follow rapid reactions: a low-cost stopped-flow

Quimica nova

Batistela, Vagner Roberto¹  Souza, Vagner Roberto de¹  Tessaro, André Luiz¹  Hioka, Noboru¹  Soares, Rafael R. da S.¹ 

[1] Universidade Estadual de Maringá, Maringá

关键词: stopped-flow;    kinetics;    fast reactions.;   

DOI  :  10.1590/S0100-40422006000500034

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The modern stopped-flowreaction analyzer has shown high efficiency and flexibility, which providesoutstanding sample economy with a dead-time of less than 1 ms. However the costof the equipment imposes a serious restriction to many Brazilian scientistsand teachers. In this work we describe the construction of a low-cost stopped-flowsystem coupled to a UV-Vis spectrophotometer. The performance of the systemwas checked by monitoring the kinetics of two reactions: the fading of phenolphthaleinin aqueous alkaline solution and the chlorophyll a demetallation in acid medium.The apparatus showed reasonable efficiency with a dead-time of 0.3 to 0.5 s.The very good results obtained in these two illustrative processes show thatthe system is satisfactory for determining rate constants with mean reactiontimes ranging from seconds to minutes.

【 授权许可】

CC BY