Culturable heterotrophic bacteria from Potter Cove, Antarctica, and their hydrolytic enzymes production

Polar research

Daniel Cicero¹  Walter Mac Cormack²  Silvia Coria⁵  Susana Vázquez⁷  Mauro Tropeano⁷  Adrián Turjanski⁸  Andrés Bercovich^1,10 

[1] Department of Biological Chemistry, School of Exact and Natural Sciences, University of Buenos Aires, Ciudad Universitaria Pabellón 21428 Buenos Aires Argentina;Department of Chemical Science and Technology, University of Rome “Tor Vergata” Via del Politecnico 1IT-00133 Rome Italy;Laboratory of Industrial Microbiology and Biotechnology, School of Pharmacy and Biochemistry, University of Buenos Aires Junín 9561113 Buenos Aires Argentina;National Scientific and Technical Research Council Rivadavia 1917, 1033Buenos Aires Argentina Correspondence;Argentine Antarctic Institute Cerrito 12481026 Buenos Aires Argentina;Argentine Antarctic Institute Cerrito 12481026 Buenos Aires Argentina;Biosidus S.A. Constitución 42341232 Buenos Aires Argentina;Department of Inorganic, Analytical and Physical Chemistry, Institute of Materials, Environment and Energy Chemistry and Physics, School of Exact and Natural Sciences, University of Buenos Aires, Ciudad Universitaria Pabellón 21428 Buenos Aires Argentina;Laboratory of Industrial Microbiology and Biotechnology, School of Pharmacy and Biochemistry, University of Buenos Aires Junín 9561113 Buenos Aires Argentina;Leloir Institute Foundation Patricias Argentinas 4351405 Buenos Aires Argentina

关键词: Microbial enzymes;    Antarctic bacteria;    marine bacteria;    cold enzymes;    psychrophiles;   

DOI  :  10.3402/polar.v31i0.18507

学科分类：自然科学（综合）

来源: Co-Action Publishing

【 摘 要 】

Affiliations of the dominant culturable bacteria isolated from Potter Cove, South Shetland Islands, Antarctica, were investigated together with their production of cold-active hydrolytic enzymes. A total of 189 aerobic heterotrophic bacterial isolates were obtained at 4°C and sorted into 63 phylotypes based on their amplified ribosomal DNA restriction analysis profiles. The sequencing of the 16S rRNA genes of representatives from each phylotype showed that the isolates belong to the phyla Proteobacteria (classes Alpha- and Gamma-proteobacteria), Bacteroidetes (class Flavobacteria), Actinobacteria (class Actinobacteria) and Firmicutes (class Bacilli). The predominant culturable group in the site studied belongs to the class Gammaproteobacteria, with 65 isolates affiliated to the genus Pseudoalteromonas and 58 to Psychrobacter. Among the 189 isolates screened, producers of amylases (9.5%), pectinases (22.8%), cellulases (14.8%), CM-cellulases (25.4%), xylanases (20.1%) and proteases (44.4%) were detected. M...

【 授权许可】

CC BY-NC