Wolbachia Infections in Aedes aegypti Differ Markedly in Their Response to Cyclical Heat Stress

PLoS Pathogens

Jason K. Axford¹  Perran A. Ross²  Nancy M. Endersby-Harshman²  Ary A. Hoffmann²  Vanessa L. White²  Itsanun Wiwatanaratanabutr² 

[1] Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand;Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia

关键词: Wolbachia;    Arboviral infections;    Larvae;    Mosquitoes;    Aedes aegypti;    Thermal stresses;    Dengue virus;    Zika virus;   

DOI  :  10.1371/journal.ppat.1006006

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Aedes aegypti mosquitoes infected with Wolbachia bacteria are currently being released for arbovirus suppression around the world. Their potential to invade populations and persist will depend on interactions with environmental conditions, particularly as larvae are often exposed to fluctuating and extreme temperatures in the field. We reared Ae. aegypti larvae infected with different types of Wolbachia (wMel, wAlbB and wMelPop-CLA) under diurnal cyclical temperatures. Rearing wMel and wMelPop-CLA-infected larvae at 26–37°C reduced the expression of cytoplasmic incompatibility, a reproductive manipulation induced by Wolbachia. We also observed a sharp reduction in the density of Wolbachia in adults. Furthermore, the wMel and wMelPop-CLA infections were not transmitted to the next generation when mosquitoes were exposed to 26–37°C across all life stages. In contrast, the wAlbB infection was maintained at a high density, exhibited complete cytoplasmic incompatibility, and was transmitted from mother to offspring with a high fidelity under this temperature cycle. These findings have implications for the potential success of Wolbachia interventions across different environments and highlight the importance of temperature control in rearing.

【 授权许可】

CC BY