Thermostable enzymes: sources, production and industrial applications

Quimica nova

Gomes, Eleni¹  Martin, Natalia¹  Silva, Roberto da¹  Guez, Marcelo Andrés Umsza¹ 

[1] Universidade Estadual Paulista, São José do Rio Preto, Brasil

关键词: thermostable enzyme;    thermophilic microorganism;    thermal adaptation.;   

DOI  :  10.1590/S0100-40422007000100025

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

REVIEW:Living organisms encountered in hostile environments that are characterizedby extreme temperatures rely on novel molecular mechanisms to enhance the thermalstability of their proteins, nucleic acids, lipids and cell membranes. Proteinsisolated from thermophilic organisms usually exhibit higher intrinsic thermalstabilities than their counterparts isolated from mesophilic organisms. Althoughthe molecular basis of protein thermostability is only partially understood,structural studies have suggested that the factors that may contribute to enhanceprotein thermostability mainly include hydrophobic packing, enhanced secondarystructure propensity, helix dipole stabilization, absence of residues sensitiveto oxidation or deamination, and increased electrostatic interactions. Thermostableenzymes such as amylases, xylanases and pectinases isolated from thermophilicorganisms are potentially of interest in the optimization of industrial processesdue to their enhanced stability. In the present review, an attempt is made todelineate the structural factors that increase enzyme thermostability and todocument the research results in the production of these enzymes.

【 授权许可】

CC BY