A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies

PLoS Pathogens

Alba Torrents de la Peña¹  John P. Moore¹  Helen J. Kim¹  Claudia Blattner¹  Anila Yasmeen¹  Andrew B. Ward¹  Albert Cupo²  Jacob Korzun²  C. Richter King²  Ronald Derking²  Michael Golabek³  Thomas J. Ketas³  Marit J. van Gils³  Rogier W. Sanders³  Natalia de Val³  Kevin de los Reyes³  Jean-Philippe Julien³  Ian A. Wilson⁴  P. J. Klasse⁵ 

[1] Department of Integrative Structural and Computational Biology, IAVI Neutralizing Antibody Center and CHAVI-ID, The Scripps Research Institute, La Jolla, California, United States of America;Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands;Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, New York, United States of America;International AIDS Vaccine Initiative, New York, New York, United States of America;The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California, United States of America

关键词: Antigens;    Enzyme-linked immunoassays;    Negative staining;    Electron microscopy;    Antibodies;    Binding analysis;    HIV-1;    Stoichiometry;   

DOI  :  10.1371/journal.ppat.1003618

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

A desirable but as yet unachieved property of a human immunodeficiency virus type 1 (HIV-1) vaccine candidate is the ability to induce broadly neutralizing antibodies (bNAbs). One approach to the problem is to create trimeric mimics of the native envelope glycoprotein (Env) spike that expose as many bNAb epitopes as possible, while occluding those for non-neutralizing antibodies (non-NAbs). Here, we describe the design and properties of soluble, cleaved SOSIP.664 gp140 trimers based on the subtype A transmitted/founder strain, BG505. These trimers are highly stable, more so even than the corresponding gp120 monomer, as judged by differential scanning calorimetry. They are also homogenous and closely resemble native virus spikes when visualized by negative stain electron microscopy (EM). We used several techniques, including ELISA and surface plasmon resonance (SPR), to determine the relationship between the ability of monoclonal antibodies (MAbs) to bind the soluble trimers and neutralize the corresponding virus. In general, the concordance was excellent, in that virtually all bNAbs against multiple neutralizing epitopes on HIV-1 Env were highly reactive with the BG505 SOSIP.664 gp140 trimers, including quaternary epitopes (CH01, PG9, PG16 and PGT145). Conversely, non-NAbs to the CD4-binding site, CD4-induced epitopes or gp41ECTO did not react with the trimers, even when their epitopes were present on simpler forms of Env (e.g. gp120 monomers or dissociated gp41 subunits). Three non-neutralizing MAbs to V3 epitopes did, however, react strongly with the trimers but only by ELISA, and not at all by SPR and to only a limited extent by EM. These new soluble trimers are useful for structural studies and are being assessed for their performance as immunogens.

【 授权许可】

CC BY