PLoS Pathogens | |
The C-Terminus of Toxoplasma RON2 Provides the Crucial Link between AMA1 and the Host-Associated Invasion Complex | |
John C. Boothroyd1  Jessica S. Tyler1  | |
[1] Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, United States of America | |
关键词: Parasitic diseases; Host cells; Glutathione chromatography; Toxoplasma; Cell membranes; Membrane proteins; Plasmodium; Immune serum; | |
DOI : 10.1371/journal.ppat.1001282 | |
学科分类:生物科学(综合) | |
来源: Public Library of Science | |
【 摘 要 】
Host cell invasion by apicomplexan parasites requires formation of the moving junction (MJ), a ring-like apposition between the parasite and host plasma membranes that the parasite migrates through during entry. The Toxoplasma MJ is a secreted complex including TgAMA1, a transmembrane protein on the parasite surface, and a complex of rhoptry neck proteins (TgRON2/4/5/8) described as host cell-associated. How these proteins connect the parasite and host cell has not previously been described. Here we show that TgRON2 localizes to the MJ and that two short segments flanking a hydrophobic stretch near its C-terminus (D3 and D4) independently associate with the ectodomain of TgAMA1. Pre-incubation of parasites with D3 (fused to glutathione S-transferase) dramatically reduces invasion but does not prevent injection of rhoptry bulb proteins. Hence, the entire C-terminal region of TgRON2 forms the crucial bridge between TgAMA1 and the rest of the MJ complex but this association is not required for rhoptry protein injection.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201902016426346ZK.pdf | 1406KB | download |