Determination of valproic acid in serum by high performance liquid chromatography with diode array detection (HPLC-DAD), after derivatization with phenacyl bromide

Quimica nova

Linden, Rafael¹  Nagel, Viviane¹  Antunes, Marina Venzon¹  Werlang, Helenice Orosco²  Hermes, Daniela² 

[1] Centro Universitário Feevale, Novo Hamburgo, Brasil;Weinmann Laboratório, Porto Alegre, Brasil

关键词: valproic acid;    phenacyl bromide;    HPLC-DAD.;   

DOI  :  10.1590/S0100-40422009000500027

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Valproic acid (VA) is a drug used to control seizures in several epileptic conditions. In VA pharmacotherapy, therapeutic drug monitoring is recommended to obtain adequate seizure control and avoid toxicity. The aim of this study was to validate a method for the determination of valproic acid in serum, employing high performance liquid chromatography with diode array detection (HPLC-DAD), after derivatization with phenacyl bromide. The calibration curve (y=0.0133x-0.0025) presented good linearity with r2=0.9999. Accuracy (101-115%), intra-assay precision (4.53-8.15%) and inter-assay precision (3.15-6.77%) were acceptable. The quantification limit was 2.0 µg/mL. The method presented similar results to enzyme immunoassay.

【 授权许可】

CC BY