A Bat-Derived Putative Cross-Family Recombinant Coronavirus with a Reovirus Gene

PLoS Pathogens

Liang Wang¹  Wei Ji²  Guang Liu²  Hongjie Yu²  Di Liu²  Qihui Wang³  Huaxing Liu³  Yingze Zhao⁴  Lin Lu⁴  Wen Xu⁵  Honghua Wen⁵  George F. Gao⁶  William J. Liu⁷  Hao Jia⁷  Canping Huang⁷  Yi Shi⁷  Yongming Zhou⁷  Hong Li⁷  Jingdong Song⁷  Edward C. Holmes⁸  Ying Wu⁹  Tao Jin⁹  Honglan Zhao⁹ 

[1]CAS Key Laboratory of Microbial and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

[2]CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

[3]Center for Disease Control and Prevention of Mengla County, Mengla Yunnan, China

[4]China National Genebank-Shenzhen, BGI-Shenzhen, Shenzhen, China

[5]College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China

[6]Division of Infectious Disease, Key Laboratory of Surveillance and Early-warning on Infectious Disease, Chinese Centre for Disease Control and Prevention, Beijing, China

[7]National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, China

[8]Network Information Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

[9]Yunnan Provincial Center for Disease Control and Prevention, Kunming Yunnan, China

关键词: Coronaviruses;    Reoviruses;    Comparative genomics;    Phylogenetic analysis;    Gene prediction;    Plasmid construction;    Recombinant proteins;    dsRNA viruses;   

DOI  :  10.1371/journal.ppat.1005883

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002 and Middle East respiratory syndrome coronavirus (MERS-CoV) in 2012 has generated enormous interest in the biodiversity, genomics and cross-species transmission potential of coronaviruses, especially those from bats, the second most speciose order of mammals. Herein, we identified a novel coronavirus, provisionally designated Rousettus bat coronavirus GCCDC1 (Ro-BatCoV GCCDC1), in the rectal swab samples of Rousettus leschenaulti bats by using pan-coronavirus RT-PCR and next-generation sequencing. Although the virus is similar to Rousettus bat coronavirus HKU9 (Ro-BatCoV HKU9) in genome characteristics, it is sufficiently distinct to be classified as a new species according to the criteria defined by the International Committee of Taxonomy of Viruses (ICTV). More striking was that Ro-BatCoV GCCDC1 contained a unique gene integrated into the 3’-end of the genome that has no homologs in any known coronavirus, but which sequence and phylogeny analyses indicated most likely originated from the p10 gene of a bat orthoreovirus. Subgenomic mRNA and cellular-level observations demonstrated that the p10 gene is functional and induces the formation of cell syncytia. Therefore, here we report a putative heterologous inter-family recombination event between a single-stranded, positive-sense RNA virus and a double-stranded segmented RNA virus, providing insights into the fundamental mechanisms of viral evolution.

【 授权许可】

CC BY