Laser raman and infrared spectrum analysis on low-density lipoproteins purified from hen egg yolk

Scientific Research and Essays

Xue Hui-jun¹ 

关键词:  ;    Low-density lipoprotein (LDL);     ;    sodium dodecyl sulfate polyacrylamide gel electrophoresis ;    (SDS-PAGE);    C-H ;    stretching vibration;    infrared spectrum;    laser raman spectrum.;   

DOI  :  10.5897/SRE11.1965

学科分类：社会科学、人文和艺术（综合）

来源: Academic Journals

【 摘 要 】

Diversified proteins were separated from hen egg yolk by ammonium sulphate rapid fractionation, and pure low-density lipoprotein (LDL) was obtained after filtrating through Sephadex G-200chromatography.After the qualitative detection ofSodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE), we discovered thatLDL consists of five major apoproteins.The results show that: (1) the CH2presents asymmetric stretching and symmetric stretching mode, (2) thederivative of cholesterol C=C stretching vibration is at 1652.07 cm-1in the Raman spectrum, and (3)a-helix bands in apolipoprotein is at 1655.12 cm-1in the infrared spectrum. The results also suggest thatlaser raman and infrared spectrum analysis on LDLcan provide useful information for studying the LDL structure.

【 授权许可】

CC BY