期刊论文详细信息
PLoS Pathogens
Inflammatory Stimuli Reprogram Macrophage Phagocytosis to Macropinocytosis for the Rapid Elimination of Pathogens
Jean Pieters1  Somdeb BoseDasgupta1 
[1] Biozentrum, University of Basel, Basel, Switzerland
关键词: Macrophages;    Mycobacteria;    Phagocytosis;    Serine;    Mycobacterium bovis;    Lysosomes;    Phosphorylation;    Immunoprecipitation;   
DOI  :  10.1371/journal.ppat.1003879
学科分类:生物科学(综合)
来源: Public Library of Science
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【 摘 要 】

Following an infectious challenge, macrophages have to be activated in order to allow efficient clearance of infectious pathogens, but how macrophage activation is coupled to increased clearance remains largely unknown. We here describe that inflammatory stimuli induced the reprogramming of the macrophage endocytic machinery from receptor-mediated phagocytosis to macropinocytosis, allowing the rapid transfer of internalized cargo to lysosomes in a receptor-independent manner. Reprogramming occurred through protein kinase C-mediated phosphorylation of the macrophage protein coronin 1, thereby activating phosphoinositol (PI)-3-kinase activity necessary for macropinocytic uptake. Expression of a phosphomimetic form of coronin 1 was sufficient to induce PI3-kinase activation and macropinocytosis even in the absence of inflammatory stimuli. Together these results suggest a hitherto unknown mechanism to regulate the internalization and degradation of infectious material during inflammation.

【 授权许可】

CC BY   

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