Leptospira spp. infection in sheep herds in southeast Brazil

Journal of venomous animals and toxins

Helio Langoni²  Virgí⁴  Priscila Barbante⁵  nia B Richini-Pereira⁵  Simone B Lucheis⁷  Fabio H Shimabukuro⁷ 

[1] CLR II, Bauru, Brazil;Univ Estadual Paulista), Botucatu, Brazil;Adolfo Lutz Institute –Adolfo Lutz Institute, Sorocaba, Brazil;Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Husbandry, SãPaulista Agency of Agribusiness Technology (APTA/SAA), Bauru, Brazil;o Paulo State University (UNESP –

关键词: Leptospirosis;    Ovine;    Serology;    Culture;    PCR;   

DOI  :  10.1186/1678-9199-20-20

学科分类：药理学

来源: BioMed Central

【 摘 要 】

With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp. MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys and four livers), corresponding to five animals with positive serology (one animal simultaneously positive for both kidney and liver) and two negatives. PCR detected Leptospira spp. in 14 samples (seven kidneys and seven livers) corresponding to 12 positive animals (two animals simultaneously positive for kidney and liver), of which ten were serologically positive and two negative. PCR was faster, more practical and more sensitive than culture for detecting leptospires. The results reinforce the importance of sheep in the epidemiological context of leptospirosis.

【 授权许可】

CC BY