Emergence and Pathogenicity of Highly Virulent Cryptococcus gattii Genotypes in the Northwest United States

PLoS Pathogens

Robert J. Bildfell¹  Wenjun Li²  Joseph Heitman²  Edmond J. Byrnes III²  Yonathan Lewit²  Hansong Ma³  Robin C. May³  Kerstin Voelz³  Dee A. Carter⁴  Ping Ren⁵  Vishnu Chaturvedi⁵ 

[1] Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, United States of America;Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America;Department of Molecular Pathobiology, School of Biosciences, University of Birmingham, Birmingham, United Kingdom;Department of Molecular and Microbial Biosciences, The University of Sydney, Sydney, New South Wales, Australia;Mycology Laboratory, Wadsworth Center, Albany, New York, United States of America

关键词: Cryptococcus gattii;    Isl;    s;    Alleles;    Mitochondria;    South America;    Cryptococcus neoformans;    Sequence analysis;    Macrophages;   

DOI  :  10.1371/journal.ppat.1000850

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Cryptococcus gattii causes life-threatening disease in otherwise healthy hosts and to a lesser extent in immunocompromised hosts. The highest incidence for this disease is on Vancouver Island, Canada, where an outbreak is expanding into neighboring regions including mainland British Columbia and the United States. This outbreak is caused predominantly by C. gattii molecular type VGII, specifically VGIIa/major. In addition, a novel genotype, VGIIc, has emerged in Oregon and is now a major source of illness in the region. Through molecular epidemiology and population analysis of MLST and VNTR markers, we show that the VGIIc group is clonal and hypothesize it arose recently. The VGIIa/IIc outbreak lineages are sexually fertile and studies support ongoing recombination in the global VGII population. This illustrates two hallmarks of emerging outbreaks: high clonality and the emergence of novel genotypes via recombination. In macrophage and murine infections, the novel VGIIc genotype and VGIIa/major isolates from the United States are highly virulent compared to similar non-outbreak VGIIa/major-related isolates. Combined MLST-VNTR analysis distinguishes clonal expansion of the VGIIa/major outbreak genotype from related but distinguishable less-virulent genotypes isolated from other geographic regions. Our evidence documents emerging hypervirulent genotypes in the United States that may expand further and provides insight into the possible molecular and geographic origins of the outbreak.

【 授权许可】

CC BY