Chronic Wasting Disease in Bank Voles: Characterisation of the Shortest Incubation Time Model for Prion Diseases

PLoS Pathogens

Joaquín Castilla¹  Jan Langeveld²  Geraldina Riccardi³  Umberto Agrimi³  Gabriele Vaccari³  Laura Pirisinu³  Michele Angelo Di Bari³  Claudia D'Agostino³  Michela Conte³  Romolo Nonno³  Robert Kunkle⁴  Juergen Richt⁴ 

[1] CIC bioGUNE and IKERBASQUE, Basque Foundation for Science, Derio and Bilbao, Bizkaia, Spain;Department of Infection Biology, Central Veterinary Institute of Wageningen UR, Lelystad, Netherlands;Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Rome, Italy;National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, United States of America

关键词: Chronic wasting disease;    Voles;    Deer;    Animal prion diseases;    Mouse models;    Mules;    Scrapie;    Sheep;   

DOI  :  10.1371/journal.ppat.1003219

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

In order to assess the susceptibility of bank voles to chronic wasting disease (CWD), we inoculated voles carrying isoleucine or methionine at codon 109 (Bv109I and Bv109M, respectively) with CWD isolates from elk, mule deer and white-tailed deer. Efficient transmission rate (100%) was observed with mean survival times ranging from 156 to 281 days post inoculation. Subsequent passages in Bv109I allowed us to isolate from all CWD sources the same vole-adapted CWD strain (Bv109ICWD), typified by unprecedented short incubation times of 25–28 days and survival times of ∼35 days. Neuropathological and molecular characterisation of Bv109ICWD showed that the classical features of mammalian prion diseases were all recapitulated in less than one month after intracerebral inoculation. Bv109ICWD was characterised by a mild and discrete distribution of spongiosis and relatively low levels of protease-resistant PrPSc (PrPres) in the same brain regions. Despite the low PrPres levels and the short time lapse available for its accumulation, end-point titration revealed that brains from terminally-ill voles contained up to 108,4 i.c. ID50 infectious units per gram. Bv109ICWD was efficiently replicated by protein misfolding cyclic amplification (PMCA) and the infectivity faithfully generated in vitro, as demonstrated by the preservation of the peculiar Bv109ICWD strain features on re-isolation in Bv109I. Overall, we provide evidence that the same CWD strain was isolated in Bv109I from the three-cervid species. Bv109ICWD showed unique characteristics of “virulence”, low PrPres accumulation and high infectivity, thus providing exceptional opportunities to improve basic knowledge of the relationship between PrPSc, neurodegeneration and infectivity.

【 授权许可】

CC BY