Differential expression of Survivin in mouse marrow-derived dendritic cells of different differentiation stages

Scientific Research and Essays

Wei-Yang He¹ 

关键词: Survivin;    dendritic cells;    immune tolerance;    mouse marrow.;   

DOI  :  10.5897/SRE10.1068

学科分类：社会科学、人文和艺术（综合）

来源: Academic Journals

【 摘 要 】

Survivin, a newly discovered member of the inhibitor of apoptosis protein (IAP) family, is over-expressed in tumor and embryonic tissues, but its expression and function in normally differentiated and maturated tissues is rarely reported. The differential expression ofsurvivinin different differentiation stages of dendritic cells (DC), which play an important role in immune tolerance, was explored in the present study. Bone marrow cells were isolated and harvested as DC precursors. Scanning electron microscopy was adopted to observe DC morphology, flow cytometry to identify DC phenotype and mixed lymphocyte reaction (MLR) to compare the ability of DC stimulating allogeneic T cell proliferation.Survivinexpression in different differentiation stages of DC was detected. Scanning electron microscopy displayed all different differentiation stages of cells showed typical DC morphology. Flow cytometry showed high expression of CD11c, a relatively specific marker of mouse marrow-derived DC(mDC); CD40, CD86 and MHC-IIshowed high expression on rmTNF-α-stimulated DC, and MLR revealed the ability of stimulating allogeneic T cell proliferation was also stronger. RT-PCR and Western-blotting assay showed that relative repressions ofsurvivinmRNA and protein in DC precursors, imDC and mDC were 0.3654±0.0702 and 0.1228±0.0162, 0.5582±0.0213 and 0.1443±0.0139, 0.7537±0.0415 and 0.3911±0.0298, respectively (P<0.05). Under different induction conditions,survivinexpression varied, and showed an ascending trend with the differentiation and maturation of DC. Inhibition ofsurvivnexpression in DC could induce immature dendritic cells (imDC) with immune tolerance.

【 授权许可】

CC BY