Feeding Cells Induced by Phytoparasitic Nematodes Require γ-Tubulin Ring Complex for Microtubule Reorganization

PLoS Pathogens

Pierre Hilson¹  Mansour Karimi¹  Jean-Luc Evrard²  Maria Teresa Mellilo³  Teresa Bleve-Zacheo³  Andrei Smertenko⁴  Bruno Favery⁵  Fabien Baldacci-Cresp⁵  Natalia Rodiuc⁵  Gilbert Engler⁵  Pierre Abad⁵  Mohamed Youssef Banora⁵  Janice de Almeida Engler⁵ 

[1] Department of Plant Systems Biology, Flanders Institute for Biotechnology, Ghent, Belgium;Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France;Istituto di Nematologia Agraria, CNR, Bari, Italia;School of Biological and Biomedical Sciences, Durham University, Durham, United Kingdom;Unité Mixte de Recherches Interactions Biotiques et Santé Végétale, INRA-CNRS-UNS, Sophia Antipolis, France

关键词: Giant cells;    Nematode infections;    Microtubules;    Arabidopsis thaliana;    Cytoplasm;    Nucleation;    Seedlings;    Cytoskeleton;   

DOI  :  10.1371/journal.ppat.1002343

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Reorganization of the microtubule network is important for the fast isodiametric expansion of giant-feeding cells induced by root-knot nematodes. The efficiency of microtubule reorganization depends on the nucleation of new microtubules, their elongation rate and activity of microtubule severing factors. New microtubules in plants are nucleated by cytoplasmic or microtubule-bound γ-tubulin ring complexes. Here we investigate the requirement of γ-tubulin complexes for giant feeding cells development using the interaction between Arabidopsis and Meloidogyne spp. as a model system. Immunocytochemical analyses demonstrate that γ-tubulin localizes to both cortical cytoplasm and mitotic microtubule arrays of the giant cells where it can associate with microtubules. The transcripts of two Arabidopsis γ-tubulin (TUBG1 and TUBG2) and two γ-tubulin complex proteins genes (GCP3 and GCP4) are upregulated in galls. Electron microscopy demonstrates association of GCP3 and γ-tubulin as part of a complex in the cytoplasm of giant cells. Knockout of either or both γ-tubulin genes results in the gene dose-dependent alteration of the morphology of feeding site and failure of nematode life cycle completion. We conclude that the γ-tubulin complex is essential for the control of microtubular network remodelling in the course of initiation and development of giant-feeding cells, and for the successful reproduction of nematodes in their plant hosts.

【 授权许可】

CC BY