Molecular detection of Leishmania spp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil

Journal of venomous animals and toxins

Rodrigo Costa da Silva¹  Virginia Bodelã¹  Enio Yoshinori Hayasaka²  Pamela Merlo Marson³  lio Langoni³  o Richini-Pereira⁴  Hé⁴  Cassiano Victoria⁵ 

[1] Departamento de Higiene Veterináblica, Faculdade de Medicina Veterináde Púria e Saúria e Zootecnia, Universidade Estadual Paulista (UNESP), Botucatu, Brasil

关键词: Road-killed animal;    Leishmania spp;    Leishmania chagasi;    PCR;    Zoonosis;   

DOI  :  10.1186/1678-9199-20-27

学科分类：药理学

来源: BioMed Central

【 摘 要 】

Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens as Leishmania spp., offering new opportunities for epidemiological studies of this infection. This study aimed to evaluate the presence of Leishmania spp. and Leishmania chagasi DNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals. DNA was detected in tissues of one Cavia aperea (Brazilian guinea pig), five Cerdocyon thous (crab-eating fox), one Dasypus septemcinctus (seven-banded armadillo), two Didelphis albiventris (white-eared opossum), one Hydrochoerus hydrochoeris (capybara), two Myrmecophaga tridactyla (giant anteater), one Procyon cancrivorus (crab-eating raccoon), two Sphiggurus spinosus (porcupine) and one Tamandua tetradactyla (lesser anteater) from different locations in the Central Western part of São Paulo state. The Leishmania chagasi DNA were confirmed in mesenteric lymph node of one Cerdocyon thous. Results indicated common infection in wild animals. The approach employed herein proved useful for detecting the environmental occurrence of Leishmania spp. and L. chagasi, as well as determining natural wild reservoirs and contributing to understand the host-parasite interaction.

【 授权许可】

CC BY