Analytical method development and validation of Doxazosin Mesylate uncoated tablets by RP-HPLC

International Journal of Biomedical Research

Shyam Sunder K¹  Harish Racha¹  M. R. Jayapal²  Santosh Kumar A Sreevatsav³ 

[1] Dept. of Pharmaceutical Chemistry, MLR Institute of Pharmacy, Dundigal (V), Hyderabad-43, A P;Institute of Aeronautical Engineering College Hyd, Affiliated to (JNTU-Hyderabad);MRR College Of Pharmacy, Nadergul, Saroornagar, R.R.Dist

关键词: Chromolith column;    Methanol;    Phosphate buffer;    Isocratic;    Stability;   

DOI  :  10.7439/ijbr.v4i9.306

学科分类：基础医学

来源: Scholar Science Journals

【 摘 要 】

A Simple, Precise, Stability indicating reverse phase HPLC method has been developed and validated in order to determine Doxazosin mesylate uncoated tablet dosage form. A Chromolith RP-18 column made up of Stainless Steel has been used with dimensions 100mm x 4.6mm x 10m. An Isocratic method with Mobile phase consisting of phosphate buffer: Methanol (40:60) ratio. A UV-Detector is used and the wavelength was set at 251nm.At the flow rate of 1.0ml/min the mobile phase was run for 10 minutes. The method was validated for Linearity, Precision, Accuracy, Robustness, and Stability of analytical solution. The Linearity range for the standard and the placebo was found to be between 50% and 150%. The method was validated for precision in method, system and intermediate. All the validation parameters have come under the limits.

【 授权许可】

CC BY