Measurement of DNA Length Changes upon CpG Hypermethylation by Microfluidic Molecular Stretching

Cell Medicine

Daisuke Onoshima¹  Yoshinobu Baba²  Jun Miyake³  Hirohiko Niioka³  Naoko Kawakita⁴  Hiroshi Yukawa⁴  Daiki Takeshita⁴ 

[1] * Institute of Innovation for Future Society, Nagoya University, Chikusa-ku, Nagoya, Japan† ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Chikusa-ku, Nagoya, Japan;* Institute of Innovation for Future Society, Nagoya University, Chikusa-ku, Nagoya, Japan† ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Chikusa-ku, Nagoya, Japan‡ Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan¶ Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan;§ Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan;† ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Chikusa-ku, Nagoya, Japan‡ Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan

关键词: DNA methylation;    Cytosine-guanine dinucleotides (CpG);    Microfluidic device;    Single-molecule detection;   

DOI  :  10.3727/215517916X693087

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

Abnormal DNA methylation in CpG-rich promoters is recognized as a distinct molecular feature of precursor lesions to cancer. Such unintended methylation can occur during in vitro differentiation of stem cells. It takes place in a subset of genes during the differentiation or expansion of stem cell derivatives under general culture conditions, which may need to be monitored in future cell transplantation studies. Here we demonstrate a microfluidic device for investigating morphological length changes in DNA methylation. Arrayed polymer chains of single DNA molecules were fluorescently observed by parallel trapping and stretching in the micro-fluidic channel. This observational study revealed that the shortened DNA length is due to the increased rigidity of the methylated DNA molecule. The trapping rate of the device for DNA molecules was substantially unaffected by changes in the CpG methylation.

【 授权许可】

CC BY