Induced Pluripotent Stem Cell Labeling Using Quantum Dots

Cell Medicine

Hiroshi Yukawa¹  Kaoru Suzuki¹  Noritada Kaji²  Yoshinobu Baba³  Tetsuya Ishikawa⁴  Tatsuya Yamada⁴  Yuki Kano⁴ 

[1] *FIRST Research Center for Innovative Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan;*FIRST Research Center for Innovative Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan‡Department of Applied Chemistry, Nagoya University, Graduate School of Engineering, Furo-cho, Chikusa-ku, Nagoya, Japan;*FIRST Research Center for Innovative Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan‡Department of Applied Chemistry, Nagoya University, Graduate School of Engineering, Furo-cho, Chikusa-ku, Nagoya, Japan§Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho, Takamatsu, Japan;†Department of Medical Technology, Nagoya University, Graduate School of Medicine, Daikominami, Higashi-ku, Nagoya, Japan

关键词: Induced pluripotent stem (iPS) cells;    Quantum dots (QDs);    Octaarginine (R8);   

DOI  :  10.3727/215517913X674270

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

Induced pluripotent stem (iPS) cells have received remarkable attention as the cell sources for clinical applications of regenerative medicine including stem cell therapy. Additionally, labeling technology is in high demand for tracing transplanted cells used in stem cell therapy. In this study, we used quantum dots (QDs), which have distinct fluorescence abilities in comparison with traditional probes, as the labeling materials and investigated whether iPS cells could be labeled with QDs with no cytotoxicity. iPS cells could not be labeled with QDs alone but required the use of cell-penetrating peptides such as octaarginine (R8). No significant cytotoxicity to iPS cells was confirmed by up to 8 nM QDs, and the iPS cells labeled with QDs maintained their undifferentiated state and pluripotency. These data suggest that QDs can be used for fluorescence labeling of iPS cells.

【 授权许可】

CC BY