Crystal Structure of Human Herpesvirus 6B Tegument Protein U14

PLoS Pathogens

Yasuko Mori¹  Hiroki Tsuruta¹  Nora F. Mahmoud²  Bochao Wang²  Mitsuhiro Nishimura²  Huamin Tang²  Daizo Hamada²  Akiko Kawabata²  Zahra Khanlari³ 

[1] Center for Applied Structural Science, Center for Collaborative Research and Technology Development, Kobe University, Kobe, Japan;Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan;Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt

关键词: Dimers (Chemical physics);    Hydrogen bonding;    Tegument proteins;    Sequence alignment;    Crystal structure;    Multiple alignment calculation;    Dimerization;    Viral structure;   

DOI  :  10.1371/journal.ppat.1005594

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The tegument protein U14 of human herpesvirus 6B (HHV-6B) constitutes the viral virion structure and is essential for viral growth. To define the characteristics and functions of U14, we determined the crystal structure of the N-terminal domain of HHV-6B U14 (U14-NTD) at 1.85 Å resolution. U14-NTD forms an elongated helix-rich fold with a protruding β hairpin. U14-NTD exists as a dimer exhibiting broad electrostatic interactions and a network of hydrogen bonds. This is first report of the crystal structure and dimerization of HHV-6B U14. The surface of the U14-NTD dimer reveals multiple clusters of negatively- and positively-charged residues that coincide with potential functional sites of U14. Three successive residues, L424, E425 and V426, which relate to viral growth, reside on the β hairpin close to the dimer's two-fold axis. The hydrophobic side-chains of L424 and V426 that constitute a part of a hydrophobic patch are solvent-exposed, indicating the possibility that the β hairpin region is a key functional site of HHV-6 U14. Structure-based sequence comparison suggests that U14-NTD corresponds to the core fold conserved among U14 homologs, human herpesvirus 7 U14, and human cytomegalovirus UL25 and UL35, although dimerization appears to be a specific feature of the U14 group.

【 授权许可】

CC BY