Protein-species quantitative venomics: looking through a crystal ball

Journal of venomous animals and toxins

Francisco Calderó¹  Bruno Lomonte³  Jorge Ruiz Encinar⁴  n-Celis⁵  Daniel Petras⁶  Juan J. Calvete⁷  Alfredo Sanz-Medel⁸ 

[1] Pharmaceutical Sciences, University of California—, Costa Rica;Department of Physical and Analytical Chemistry, University of Oviedo, Oviedo, Spain;Instituto Clodomiro Picado, Facultad de MicrobiologíSan Diego, La Jolla, USA;Skaggs School of Pharmacy &Structural and Functional Venomics Laboratory, Instituto de Biomedicina de Valencia, C.S.I.C, Valencia, Spain;a, Universidad de Costa Rica, San José

关键词: Snake venomics;    Top-down proteomics;    Top-down venomics;    Protein species-resolved venomics;    Absolute quantification;    Inductively coupled plasma mass spectrometry;   

DOI  :  10.1186/s40409-017-0116-9

学科分类：药理学

来源: BioMed Central

【 摘 要 】

In this paper we discuss recent significant developments in the field of venom research, specifically the emergence of top-down proteomic applications that allow achieving compositional resolution at the level of the protein species present in the venom, and the absolute quantification of the venom proteins (the term “protein species” is used here to refer to all the different molecular forms in which a protein can be found. Please consult the special issue of Jornal of Proteomics “Towards deciphering proteomes via the proteoform, protein speciation, moonlighting and protein code concepts” published in 2016, vol. 134, pages 1-202). Challenges remain to be solved in order to achieve a compact and automated platform with which to routinely carry out comprehensive quantitative analysis of all toxins present in a venom. This short essay reflects the authors’ view of the immediate future in this direction for the proteomic analysis of venoms, particularly of snakes.

【 授权许可】

CC BY