Er:YAG Laser and Cyclosporin A Effect on Cell Cycle Regulation of Human Gingival Fibroblast Cells

Journal of Lasers in Medical Sciences

Mona Zamanian-Azodi¹  Hojjat-Allah Abbaszadeh²  Yousef Sadeghi²  Maryam Sadat Khoramgah³  Ali Asghar Peyvandi³  Akram Safaei⁴  Mostafa Rezaei-Tavirani⁴ 

[1] 3Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Hearing Disorders Research Center and Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Hearing Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

关键词: Er:YAG laser;    cyclosporin A (CsA);    Protein-protein interaction (PPI) network analysis;    Gene ontology;    human fibroblast cell.;   

DOI  :  10.22037/2010.v8i3.16363

学科分类：基础医学

来源: Shahid Beheshti University of Medical Sciences

【 摘 要 】

Introduction: Periodontitis is a set of inflammatory disorders characterized by periodontal attachment loss and alveolar bone resorption. Because of deficiency in periodontitis mechanical therapy, this study was aimed to explore the molecular influence of the erbium-doped: yttrium aluminum garnet (Er:YAG) laser and cyclosporin A (CsA) on human gingival fibroblasts (HGFs) for improvement in periodontal diseases therapy. Methods: We focused on articles that studied the proteome profiles of HGFs after treatment with laser irradiation and application of CsA. The topological features of differentially expressed proteins were analyzed using Cytoscape Version 3.4.0 followed by module selection from the protein-protein interaction (PPI) network using Cluster ONE plugin. In addition, we performed gene ontology (GO) enrichment analysis for the densely connected region and key proteins in both PPI networks. Results: Analysis of PPI network of Er:YAG laser irradiation on HGFs lead to introducing YWHAZ, VCP, HNRNPU, YWHAE, UBA52, CLTC, FUS and IGHG1 as key proteins while similar analysis revealed that ACAT1, CTSD, ALDOA, ANXA2, PRDX1, LGALS3, ARHGDI and EEF1A1 are the crucial proteins related to the effect of drug. GO enrichment analysis of hub-bottleneck proteins of the 2 networks showed the different significant biological processes and cellular components. The functional enrichments of module of Er:YAG laser network are included as fatty acid transmembrane transport, cytokinesis, regulation of RNA splicing and asymmetric protein localization. There are not any significant clusters in network of HGF treated by CsA. Conclusion: The results indicate that there are 2 separate biomarker panels for the 2 treatment methods.

【 授权许可】

CC BY