Method validation to hmf determination in honey by HPLC-UV and its influence on the product quality

Quimica nova

Lemos, Gisele da Silveira¹  Santos, José Soares dos¹  Santos, Maria Lúcia Pires dos¹ 

[1] Universidade Estadual do Sudoeste da Bahia, Vitória da Conquista, Brasil

关键词: HMF;    honey;    HPLC-UV.;   

DOI  :  10.1590/S0100-40422010000800012

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

In honey 5-hydroxymethyl-2-furaldehyde(HMF) is one of the most typical products of degradation: it is usually absentin fresh honey, but its concentration tends to rise as a result of heating processesor long-term storage. The validation protocol was performed in terms of detectionand quantification limits, precision (by repeatability and intermediate precision),linearity and accuracy (by recovery tests). The method has been tested on 15honey samples of different ages and geographical origin. HMF correlated highlywith the age of the samples has been considered a very important parameter toput these honeys on the market or not and/or to estimate their shelf life.

【 授权许可】

CC BY