Acute Treatment with Herbal Extracts Provides Neuroprotective Benefits in in Vitro and in vivo Stroke Models, Characterized by Reduced Ischemic Cell Death and Maintenance of Motor and Neurological Functions

Cell Medicine

Yuji Kaneko¹  Seongjin Yu¹  Cesar V. Borlongan¹  David J. Eve¹  Eunkyung Cate Bae¹  Paul R. Sanberg¹  Paula C. Bickford²  Hideki Shojo³  Dong-Hyuk Park⁴  Bill Roschek, Jr.⁵  Randall S. Alberte⁵  Cyndy D. Sanberg⁶ 

[1] *Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa, FL, USA;*Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa, FL, USA#James A. Haley Veterans Adminstration Hospital, Tampa FL, USA;*Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa, FL, USA†Department of Legal Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan;*Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa, FL, USA‡Department of Neurosurgery, Korea University Medical Center, Korea University College of Medicine, Seoul, Korea;§HerbalScience Group LLC, Naples, FL, USA;¶Natura Therapeutics, Inc., Tampa, FL, USA

关键词: Oxygen-glucose deprivation (OGD);    Stroke;    Prophylactic;    Middle cerebral artery occlusion (MCAo);    Plant extracts;   

DOI  :  10.3727/215517910X552818

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

The present study explored the prophylactic and restorative benefits of cacao and red sage using both in vitro and in vivo models of stroke. For the in vitro study, we initially exposed primary rat cells to the established oxygen-glucose deprivation (OGD) stroke model followed by reperfusion under normoxic conditions, then added different cacao and sage concentrations to the cell culture media. Trypan blue cell viability results revealed specific cacao and sage dosages exerted significant therapeutic effects against OGD-induced cell death compared to cultured cells treated with extract vehicle. We next embarked on testing the therapeutic effects of cacao and sage in an in vivo model of stroke when extract treatment commenced either prior to or after transient middle cerebral artery occlusion (MCAo). Significant reduction in ischemic cell death within the peri-infarct area coupled with better performance in routine motor and neurological tasks were demonstrated by stroke animals that received cacao or sage extracts prior to MCAo compared to those that received the extracts or vehicle after MCAo. In summary, the present results demonstrate that neuroprotective effects were afforded by plant extract treatment, and that the in vitro stroke paradigm approximates in vivo efficacy when considering prophylactic treatment for stroke.

【 授权许可】

CC BY