Screening of organic supports and activation protocols for immobilization and stabilization of lipase from Thermomyces lanuginosus

Quimica nova

Giordano, Raquel de L. C.¹  Mendes, Adriano A.²  Castro, Heizir F. de³ 

[1] Universidade Federal de São Carlos, São Carlos, Brasil;Universidade Federal de São João Del-Rei, Sete Lagoas, Brasil;Universidade de São Paulo, Lorena, Brasil

关键词: covalent attachment;    lipase;    organic supports.;   

DOI  :  10.1590/S0100-40422013000200008

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Lipase from Thermomyces lanuginosus was covalently immobilized on activated poly-hydroxybutyrate, sugarcane bagasse and the chemically modified hybrid hydrogel chitosan-alginate prepared by different strategies. Among the tested supports, chitosan-alginate chemically modified with 2,4,6-trinitrobenzenesulfonic acid rendered derivatives with the highest hydrolytic activity and thermal-stability, 45-fold more stable than soluble lipase and was then selected for further studies. The pH of maximum activity was similar for both immobilized and free lipase (pH 8.0) while optimum temperature was 5 - 10 ºC higher for the immobilized lipase. Higher yields in the butyl butyrate synthesis were found for the derivatives prepared by activation with glycidol and epichlorohydrin.

【 授权许可】

CC BY