First Molecular Report and Phylogenetic Analysis of Crinoidea from Rameswaram Island, South East Coast of India

Annals of Biological Research

Nina Tabitha S¹ 

关键词: Nina Tabitha S;    Gunalan B;    Crinoidea;    Echinoderm;    Phylogeny;    Cenometra sp;    Tropiometra sp;    Comatella sp;    Molecular taxonomy;   

DOI  :  

学科分类：生物科学（综合）

来源: Scholars Research Library

【 摘 要 】

Crinoidea is a class of echinoderms that degenerate very easily due to the presence of very temperature sensitive proteins, hence the samples obtained could not be identified easily, resulting in the identification of the samples at a molecular level by sequencing the 650 –bp region from the 5’ end of the mitochondrial CO1 region. The sequenced genes were identified as three different species of Crinoids; Cenometra sp, Tropiometra sp and Comatella sp submitted to the National center of bioinformatics (NCBI) which provided the three finds with accession numbers. The sequences were compared with other closely related sequences and analyzed using the CLUSTAL X software to attain a multiple sequence alignment and Mega (Molecular Evolutionary Genetic Analysis) to construct a Phylogenic tree through which the evolutionary relationships of the three samples analyzed could be observed in the study.

【 授权许可】

CC BY