Characteristics of hemolytic activity induced by skin secretions of the frog Kaloula pulchra hainana

Journal of venomous animals and toxins

Yingxia Zhang¹  Tingting Chi¹  Tianchen An²  Shuangshuang Wei²  Manchuriga Wang²  Congwei Chen²  Aiyun Meng² 

[1] College of Agriculture, Hainan University, Haikou, China;Key Laboratory of Tropic Biological Resources, Minister of Education, College of Marine Science, Hainan University, Haikou, China

关键词: Amphibian;    Skin secretions;    Hemolysis;    Pore-forming;   

DOI  :  10.1186/1678-9199-19-9

学科分类：药理学

来源: BioMed Central

【 摘 要 】

The hemolytic activity of skin secretions obtained by stimulating the frog Kaloula pulchra hainana with diethyl ether was tested using human, cattle, rabbit, and chicken erythrocytes. The skin secretions had a significant concentration-dependent hemolytic effect on erythrocytes. The hemolytic activity of the skin secretions was studied in the presence of osmotic protectants (polyethylene glycols and carbohydrates), cations (Mg2+, Ca2+, Ba2+, Cu2+, and K+), or antioxidants (ascorbic acid, reduced glutathione, and cysteine). Depending on their molecular mass, osmotic protectants effectively inhibited hemolysis. The inhibition of skin hemolysis was observed after treatment with polyethylene glycols (1000, 3400, and 6000 Da). Among divalent cations, only 1 mM Cu2+ markedly inhibited hemolytic activity. Antioxidant compounds slightly reduced the hemolytic activity. The results suggested that skin secretions of K. pulchra hainana induce a pore-forming mechanism to form pores with a diameter of 1.36-2.0 nm rather than causing oxidative damage to the erythrocyte membrane.

【 授权许可】

CC BY