Determination of antitryptic activity in proteins from peanut products isolated by affinity chromatography

Quimica nova

Alves, Jussara Cristina¹  Machado, Richele Janaína de Araújo¹  Neves, Renata Alexandra Moreira das¹  Serquiz, Alexandre Coelho¹  Morais, Ana Heloneida de Araújo¹  Medeiros, Amanda Fernandes de¹  Santos, Elizeu Antunes dos¹  Peixoto, Thayane Kerbele Oliveira das Neves¹  Araújo, Jéssica Morais de¹  Uchôa, Adriana Ferreira¹ 

[1] Universidade Federal do Rio Grande do Norte, Natal, Brasil

关键词: Arachis hypogaea L.;    japanese peanut;    peanut nougat;    peanut butter;    trypsin inhibitor.;   

DOI  :  10.5935/0100-4042.20140254

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The peanut is an oleaginous plant of high nutritional value, a source of protein and a trypsin inhibitor. Trypsin inhibitors are proteins present in the vegetable kingdom, considered anti-nutritional factors for animals. However, there have been several recent reports about their heterologous and beneficial effects on human health. These important effects have been the focus of studies investigating these inhibitors in foods. The aim of the present study was to isolate and determine the estimated molecular mass and specific inhibitory activity, for trypsin in the Japanese peanut, peanut butter, and peanut nougat using the techniques of precipitation with ammonium sulfate and affinity chromatography on trypsin - Sepharose CNBr 4B. The techniques used in this study were efficient for isolating the protein inhibitors with antitryptic specific activity of 694 UI mg-1, 823 UI mg-1 and 108 UI mg-1 for the Japanese peanut, peanut nougat, and peanut butter, respectively. The techniques featured high selectivity of the adsorbent, with consequent efficiency in isolation, given the low amount of dosed proteins and specific antitryptic activity presented by the products studied. The various health-related benefits show the importance of detecting and isolating efficient trypsin inhibitors in foods, taking into account the health claims attributed to the vegetable and its high consumption by humans.

【 授权许可】

CC BY