Pre-Micro RNA Signatures Delineate Stages of Endothelial Cell Transformation in Kaposi Sarcoma

PLoS Pathogens

Blossom Damania¹  Dirk P. Dittmer¹  Ling Wang¹  Andrea J. O'Hara¹  Pauline Chugh¹  Bruce J. Dezube²  Estrella Luz³  Eduardo M. Netto³  William J. Harrington Jr.⁴ 

[1] Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research at the University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America;Division of Hematology/Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America;Federal University of Bahia, Salvador, Bahia, Brazil;The Viral Oncology Program, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, Florida, United States of America

关键词: MicroRNAs;    Endothelial cells;    Kaposi sarcoma;    Biopsy;    Biomarkers;    Kaposi's sarcoma-associated herpesvirus;    Tonsils;    Messenger RNA;   

DOI  :  10.1371/journal.ppat.1000389

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

MicroRNAs (miRNA) have emerged as key regulators of cell lineage differentiation and cancer. We used precursor miRNA profiling by a novel real-time QPCR method (i) to define progressive stages of endothelial cell transformation cumulating in Kaposi sarcoma (KS) and (ii) to identify specific miRNAs that serve as biomarkers for tumor progression. We were able to compare primary patient biopsies to well-established culture and mouse tumor models. Loss of mir-221 and gain of mir-15 expression demarked the transition from merely immortalized to fully tumorigenic endothelial cells. Mir-140 and Kaposi sarcoma–associated herpesvirus viral miRNAs increased linearly with the degree of transformation. Mir-24 emerged as a biomarker specific for KS.

【 授权许可】

CC BY