Hepatitis C Virus Reveals a Novel Early Control in Acute Immune Response

PLoS Pathogens

Stéphanie Dabo¹  Noëlla Arnaud¹  Eliane F. Meurs¹  Jacques Hugon²  Fumiko Shinkai-Ouchi³  Masayoshi Fukasawa³  Daisuke Akazawa⁴  Takaji Wakita⁴ 

[1] Institut Pasteur, Hepacivirus and Innate Immunity, Paris, France;Institut du Fer à Moulin, INSERM UMRS 839, Paris, France;National Institute of Infectious Diseases, Department of Biochemistry and Cell Biology, Tokyo, Japan;National Institute of Infectious Diseases, Department of Virology II, Tokyo, Japan

关键词: Small interfering RNAs;    Hepatitis C virus;    Ubiquitination;    Interferons;    RNA extraction;    Immunoprecipitation;    Protein extraction;    Sendai virus;   

DOI  :  10.1371/journal.ppat.1002289

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Recognition of viral RNA structures by the intracytosolic RNA helicase RIG-I triggers induction of innate immunity. Efficient induction requires RIG-I ubiquitination by the E3 ligase TRIM25, its interaction with the mitochondria-bound MAVS protein, recruitment of TRAF3, IRF3- and NF-κB-kinases and transcription of Interferon (IFN). In addition, IRF3 alone induces some of the Interferon-Stimulated Genes (ISGs), referred to as early ISGs. Infection of hepatocytes with Hepatitis C virus (HCV) results in poor production of IFN despite recognition of the viral RNA by RIG-I but can lead to induction of early ISGs. HCV was shown to inhibit IFN production by cleaving MAVS through its NS3/4A protease and by controlling cellular translation through activation of PKR, an eIF2α-kinase containing dsRNA-binding domains (DRBD). Here, we have identified a third mode of control of IFN induction by HCV. Using HCVcc and the Huh7.25.CD81 cells, we found that HCV controls RIG-I ubiquitination through the di-ubiquitine-like protein ISG15, one of the early ISGs. A transcriptome analysis performed on Huh7.25.CD81 cells silenced or not for PKR and infected with JFH1 revealed that HCV infection leads to induction of 49 PKR-dependent genes, including ISG15 and several early ISGs. Silencing experiments revealed that this novel PKR-dependent pathway involves MAVS, TRAF3 and IRF3 but not RIG-I, and that it does not induce IFN. Use of PKR inhibitors showed that this pathway requires the DRBD but not the kinase activity of PKR. We then demonstrated that PKR interacts with HCV RNA and MAVS prior to RIG-I. In conclusion, HCV recruits PKR early in infection as a sensor to trigger induction of several IRF3-dependent genes. Among those, ISG15 acts to negatively control the RIG-I/MAVS pathway, at the level of RIG-I ubiquitination.These data give novel insights in the machinery involved in the early events of innate immune response.

【 授权许可】

CC BY