KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection

PLoS Pathogens

Matthias Majetschak¹  Abhishek Tripathi¹  João I. Mamede²  Thomas J. Hope²  Adarsh Dharan³  Edward M. Campbell³  Sarah Talley⁴ 

[1] Burn and Shock Trauma Research Institute, Department of Surgery, Stritch School of Medicine, Loyola University, Chicago, Illinois, United States of America;Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America;Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University, Chicago, Illinois, United States of America;Integrative Cell Biology Program, Stritch School of Medicine, Loyola University, Chicago, Illinois, United States of America

关键词: HIV-1;    Viral core;    Small interfering RNAs;    HeLa cells;    Cytoplasm;    Nuclear import;    Virions;    Viral packaging;   

DOI  :  10.1371/journal.ppat.1005700

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Following envelope mediated fusion, the HIV-1 core is released into the cytoplasm of the target cell and undergoes a series of trafficking and replicative steps that result in the nuclear import of the viral genome, which ultimately leads to the integration of the proviral DNA into the host cell genome. Previous studies have found that disruption of microtubules, or depletion of dynein or kinesin motors, perturb the normal uncoating and trafficking of the viral genome. Here, we show that the Kinesin-1 motor, KIF5B, induces a relocalization of the nuclear pore component Nup358 into the cytoplasm during HIV-1 infection. This relocalization of NUP358 is dependent on HIV-1 capsid, and NUP358 directly associates with viral cores following cytoplasmic translocation. This interaction between NUP358 and the HIV-1 core is dependent on multiple capsid binding surfaces, as this association is not observed following infection with capsid mutants in which a conserved hydrophobic binding pocket (N74D) or the cyclophilin A binding loop (P90A) is disrupted. KIF5B knockdown also prevents the nuclear entry and infection by HIV-1, but does not exert a similar effect on the N74D or P90A capsid mutants which do not rely on Nup358 for nuclear import. Finally, we observe that the relocalization of Nup358 in response to CA is dependent on cleavage protein and polyadenylation factor 6 (CPSF6), but independent of cyclophilin A. Collectively, these observations identify a previously unappreciated role for KIF5B in mediating the Nup358 dependent nuclear import of the viral genome during infection.

【 授权许可】

CC BY