Identification of GBV-D, a Novel GB-like Flavivirus from Old World Frugivorous Bats (Pteropus giganteus) in Bangladesh

PLoS Pathogens

Michael Egholm¹  Stephen P. Luby¹  Sean Conlan²  Craig Street²  Omar Jabado²  Phenix-Lan Quan²  Thomas Briese²  M. Jahangir Hossain²  Dawn Verdugo³  Jonathan H. Epstein⁴  Shahneaz Ali Khan⁴  W. Ian Lipkin⁴  Stephen K. Hutchison⁵  Peter Daszak⁵ 

[1] 454 Life Sciences, Branford, Connecticut, United States of America;Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America;Chittagong Veterinary & Animal Sciences University, Chittagong, Bangladesh;Conservation Medicine Program, Wildlife Trust, New York, New York, United States of America;Programme on Infectious Disease and Vaccine Sciences, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR,B), Dhaka, Bangladesh

关键词: Bats;    Sequence motif analysis;    Hepatitis G virus;    Hepatitis C virus;    RNA extraction;    Sequence analysis;    Polymerase chain reaction;    Zoonoses;   

DOI  :  10.1371/journal.ppat.1000972

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Bats are reservoirs for a wide range of zoonotic agents including lyssa-, henipah-, SARS-like corona-, Marburg-, Ebola-, and astroviruses. In an effort to survey for the presence of other infectious agents, known and unknown, we screened sera from 16 Pteropus giganteus bats from Faridpur, Bangladesh, using high-throughput pyrosequencing. Sequence analyses indicated the presence of a previously undescribed virus that has approximately 50% identity at the amino acid level to GB virus A and C (GBV-A and -C). Viral nucleic acid was present in 5 of 98 sera (5%) from a single colony of free-ranging bats. Infection was not associated with evidence of hepatitis or hepatic dysfunction. Phylogenetic analysis indicates that this first GBV-like flavivirus reported in bats constitutes a distinct species within the Flaviviridae family and is ancestral to the GBV-A and -C virus clades.

【 授权许可】

CC BY