期刊论文详细信息
PLoS Pathogens
Stage Specific Assessment of Candida albicans Phagocytosis by Macrophages Identifies Cell Wall Composition and Morphogenesis as Key Determinants
Lars-Peter Erwig1  Neil A. R. Gow1  Leanne E. Lewis2  Collette Gillespie2  Judith M. Bain2  Christina Lowes2  Fiona M. Rudkin2 
[1] Aberdeen Fungal Group, University of Aberdeen, Aberdeen, United Kingdom;Division of Applied Medicine, University of Aberdeen, Aberdeen, United Kingdom
关键词: C;    ida albicans;    Macrophages;    Phagocytosis;    Glycosylation;    Cell walls;    Morphogenesis;    Video microscopy;    Fungal pathogens;   
DOI  :  10.1371/journal.ppat.1002578
学科分类:生物科学(综合)
来源: Public Library of Science
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【 摘 要 】

Candida albicans is a major life-threatening human fungal pathogen. Host defence against systemic Candida infection relies mainly on phagocytosis of fungal cells by cells of the innate immune system. In this study, we have employed video microscopy, coupled with sophisticated image analysis tools, to assess the contribution of distinct C. albicans cell wall components and yeast-hypha morphogenesis to specific stages of phagocytosis by macrophages. We show that macrophage migration towards C. albicans was dependent on the glycosylation status of the fungal cell wall, but not cell viability or morphogenic switching from yeast to hyphal forms. This was not a consequence of differences in maximal macrophage track velocity, but stems from a greater percentage of macrophages pursuing glycosylation deficient C. albicans during the first hour of the phagocytosis assay. The rate of engulfment of C. albicans attached to the macrophage surface was significantly delayed for glycosylation and yeast-locked morphogenetic mutant strains, but enhanced for non-viable cells. Hyphal cells were engulfed at a slower rate than yeast cells, especially those with hyphae in excess of 20 µm, but there was no correlation between hyphal length and the rate of engulfment below this threshold. We show that spatial orientation of the hypha and whether hyphal C. albicans attached to the macrophage via the yeast or hyphal end were also important determinants of the rate of engulfment. Breaking down the overall phagocytic process into its individual components revealed novel insights into what determines the speed and effectiveness of C. albicans phagocytosis by macrophages.

【 授权许可】

CC BY   

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