期刊论文详细信息
PLoS Pathogens
Leptomonas seymouri: Adaptations to the Dixenous Life Cycle Analyzed by Genome Sequencing, Transcriptome Profiling and Co-infection with Leishmania donovani
Fred Opperdoes1  Petr Volf2  Jan Votýpka2  Danyil Grybchuk2  Pavel Flegontov2  Jana Hlaváčová2  Vyacheslav Yurchenko3  Anzhelika Butenko3  Alexei Kostygov3  Tereza Leštinová3  Natalya Kraeva3  Jitka Myškova4  Julius Lukeš5 
[1] Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic;Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic;Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic;Zoological Institute of the Russian Academy of Sciences, St. Petersburg, Russia;de Duve Institute and Université catholique de Louvain, Brussels, Belgium
关键词: Leishmania;    Leishmania donovani;    Trypanosoma;    S;    flies;    Parasitic diseases;    Vertebrates;    Macrophages;    Co-infections;   
DOI  :  10.1371/journal.ppat.1005127
学科分类:生物科学(综合)
来源: Public Library of Science
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【 摘 要 】

The co-infection cases involving dixenous Leishmania spp. (mostly of the L. donovani complex) and presumably monoxenous trypanosomatids in immunocompromised mammalian hosts including humans are well documented. The main opportunistic parasite has been identified as Leptomonas seymouri of the sub-family Leishmaniinae. The molecular mechanisms allowing a parasite of insects to withstand elevated temperature and substantially different conditions of vertebrate tissues are not understood. Here we demonstrate that L. seymouri is well adapted for the environment of the warm-blooded host. We sequenced the genome and compared the whole transcriptome profiles of this species cultivated at low and high temperatures (mimicking the vector and the vertebrate host, respectively) and identified genes and pathways differentially expressed under these experimental conditions. Moreover, Leptomonas seymouri was found to persist for several days in two species of Phlebotomus spp. implicated in Leishmania donovani transmission. Despite of all these adaptations, L. seymouri remains a predominantly monoxenous species not capable of infecting vertebrate cells under normal conditions.

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