The Combined Effect of Environmental and Host Factors on the Emergence of Viral RNA Recombinants

PLoS Pathogens

Hannah M. Jaag¹  Peter D. Nagy¹ 

[1] Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, Kentucky, United States of America

关键词: Yeast;    RNA viruses;    Northern blot;    RNA extraction;    Yeast;    fungal models;    DNA recombination;    Leaves;    Polymerase chain reaction;   

DOI  :  10.1371/journal.ppat.1001156

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Viruses are masters of evolution due to high frequency mutations and genetic recombination. In spite of the significance of viral RNA recombination that promotes the emergence of drug-resistant virus strains, the role of host and environmental factors in RNA recombination is poorly understood. Here we report that the host Met22p/Hal2p bisphosphate-3′-nucleotidase regulates the frequency of viral RNA recombination and the efficiency of viral replication. Based on Tomato bushy stunt virus (TBSV) and yeast as a model host, we demonstrate that deletion of MET22 in yeast or knockdown of AHL, SAL1 and FRY1 nucleotidases/phosphatases in plants leads to increased TBSV recombination and replication. Using a cell-free TBSV recombination/replication assay, we show that the substrate of the above nucleotidases, namely 3′-phosphoadenosine-5′-phosphate pAp, inhibits the activity of the Xrn1p 5′-3′ ribonuclease, a known suppressor of TBSV recombination. Inhibition of the activity of the nucleotidases by LiCl and NaCl also leads to increased TBSV recombination, demonstrating that environmental factors could also affect viral RNA recombination. Thus, host factors in combination with environmental factors likely affect virus evolution and adaptation.

【 授权许可】

CC BY