Method validation for the determination of volatile fatty acids in wastewaters from anaerobic reactors employing liquid chromatography

Quimica nova

Dias, Adriana Neves¹  Primel, Ednei Gilberto¹  Santana, Fabrício Butierres¹  D'Oca, Marcelo Gonçalves Montes¹  Caldas, Sergiane Souza¹  Cerqueira, Maristela Barnes Rodrigues¹ 

[1] Universidade Federal do Rio Grande, Rio Grande, Brasil

关键词: VFAs;    matrix effect;    HPLC-DAD.;   

DOI  :  10.1590/S0100-40422011000100029

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

This work deals with the method validation for the determination of acetic, propionic and butyric acids (VFAs) in wastewaters from anaerobic reactors by HPLC-DAD. Separation was performed using a C18 column and the mobile phase composition were water pH 3.0 and methanol 90:10 (v/v). The detection and quantification was carried out at 220 nm. The method shows good linearity (r2>0.996), with adequate accuracy (89-102%) and relative standard deviations lower than 18%. The matrix effect was considered low (-4.1, -3.9 and 1.4%). The developed method is fast, simple and cheap; and it was applied in wastewater samples from anaerobic reactor.

【 授权许可】

CC BY