Determination of phenolic compounds by isocratic high performance liquid chromatografic method during storage of yerba-mate

Quimica nova

Ribani, Marcelo¹  Dutra, Fabiana L. Goularte²  Hoffmann-Ribani, Rosemary² 

[1]Instituto de Tecnologia do Paraná, Curitiba, Brasil

[2]Universidade Federal do Paraná, Curitiba, Brasil

关键词: HPLC analysis;    phenolic compounds;    Ilex paraguariensis.;   

DOI  :  10.1590/S0100-40422010000100022

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Different phenoliccompound, 5- caffeoylquinic acid (5-CQA), caffeic acid (AC) and rutin (Ru) contentsof yerba-mate (Ilex paraguariensis) Brazilian samples of 06 differentregions of São Mateus - Paraná, during natural and acceleratedindustrial storage, were evaluated. For quantification, a reverse phase HPLCisocratic method was developed and validated using methanol:water (35:65 v/v)acidified with 0.5% acetic acid as mobile phase and a photodiode array detector.The six sample global average contents were (34.90 and 36.10 mg g-1)for 5-CQA, (0.18 mg g-1 and 0.23 mg g-1) for AC and (7.12and 7.18 mg g-1) for Ru, respectively, for the natural and acceleratedstorage systems. The results showed that the 5-CQA and Ru content are kept constantduring the storage while AC content increase only during accelerated storage.

【 授权许可】

CC BY