Selection and Spread of Artemisinin-Resistant Alleles in Thailand Prior to the Global Artemisinin Resistance Containment Campaign

PLoS Pathogens

Kanungnit Congpuong¹  Sheila Akinyi Okoth¹  Lindsay Morton²  Venkatachalam Udhayakumar³  Mateusz M. Plucinski³  John W. Barnwell⁴  Patrick S. Kachur⁵  Chansuda Wongsrichanalai⁵  Wichai Satimai⁵  Ira F. Goldman⁵  Eldin Talundzic⁵ 

[1] Atlanta Research and Education Foundation, Atlanta VA Medical Center, Atlanta, Georgia, United States of America;Bansomdej-chaopraya Rajabhat University, Bangkok, Thailand;Bureau of Vector Borne Diseases, Ministry of Public Health, Nonthaburi, Thailand;Independent Scholar, Bangkok, Thailand;Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America

关键词: Thail;    Genetic loci;    Malaria;    Antimicrobial resistance;    Malarial parasites;    Mutation;    Alleles;    Cambodia;   

DOI  :  10.1371/journal.ppat.1004789

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

The recent emergence of artemisinin resistance in the Greater Mekong Subregion poses a major threat to the global effort to control malaria. Tracking the spread and evolution of artemisinin-resistant parasites is critical in aiding efforts to contain the spread of resistance. A total of 417 patient samples from the year 2007, collected during malaria surveillance studies across ten provinces in Thailand, were genotyped for the candidate Plasmodium falciparum molecular marker of artemisinin resistance K13. Parasite genotypes were examined for K13 propeller mutations associated with artemisinin resistance, signatures of positive selection, and for evidence of whether artemisinin-resistant alleles arose independently across Thailand. A total of seven K13 mutant alleles were found (N458Y, R539T, E556D, P574L, R575K, C580Y, S621F). Notably, the R575K and S621F mutations have previously not been reported in Thailand. The most prevalent artemisinin resistance-associated K13 mutation, C580Y, carried two distinct haplotype profiles that were separated based on geography, along the Thai-Cambodia and Thai-Myanmar borders. It appears these two haplotypes may have independent evolutionary origins. In summary, parasites with K13 propeller mutations associated with artemisinin resistance were widely present along the Thai-Cambodia and Thai-Myanmar borders prior to the implementation of the artemisinin resistance containment project in the region.

【 授权许可】

CC BY