In Vitro and In Vivo Isolation and Characterization of Duvenhage Virus

PLoS Pathogens

Pieter-Paul A. M. van Thiel¹  Albert D. M. E. Osterhaus²  Thijs Kuiken²  Penelope Koraka²  Geert van Amerongen²  Byron E. E. Martina²  Jouke M. Roose² 

[1] Department of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands;Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands

关键词: Rabies virus;    Spinal cord;    Brainstem;    Cerebellum;    Cell staining;    Cerebrum;    Neurons;    Pathogenesis;   

DOI  :  10.1371/journal.ppat.1002682

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

A fatal human case of Duvenhage virus (DUVV) infection in a Dutch traveller who had returned from Kenya was reported in 2007. She exhibited classical symptoms of rabies encephalitis with distinct pathological findings. In the present study we describe the isolation and characterization of DUVV in vitro and its passage in BALB/c mice. The virus proved to be neuroinvasive in both juvenile and adult mice, resulting in about 50% lethality upon peripheral infection. Clinical signs in infected mice were those of classical rabies. However, the distribution of viral antigen expression in the brain differed from that of classical rabies virus infection and neither inclusion bodies nor neuronal necrosis were observed. This is the first study to describe the in vitro and in vivo isolation and characterization of DUVV.

【 授权许可】

CC BY