Exosome secretion affects social motility in Trypanosoma brucei

PLoS Pathogens

Dror Eliaz¹  Hadassa Shaked¹  Lior Binder¹  Vaibhav Chikne¹  Gil Arvatz¹  Smadar Cohen-Chalamish¹  Shulamit Michaeli¹  Sriram Kannan¹  Itai Dov Tkacz¹  Hiba Waldman Ben-Asher¹  Uthman Okalang¹ 

[1]The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University, Ramat-Gan Israel

关键词: Exosomes;    Trypanosoma;    Secretion;    Vesicles;    Small nucleolar RNAs;    Parasitic diseases;    DAPI staining;    Trypanosoma brucei gambiense;   

DOI  :  10.1371/journal.ppat.1006245

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Extracellular vesicles (EV) secreted by pathogens function in a variety of biological processes. Here, we demonstrate that in the protozoan parasite Trypanosoma brucei, exosome secretion is induced by stress that affects trans-splicing. Following perturbations in biogenesis of spliced leader RNA, which donates its spliced leader (SL) exon to all mRNAs, or after heat-shock, the SL RNA is exported to the cytoplasm and forms distinct granules, which are then secreted by exosomes. The exosomes are formed in multivesicular bodies (MVB) utilizing the endosomal sorting complexes required for transport (ESCRT), through a mechanism similar to microRNA secretion in mammalian cells. Silencing of the ESCRT factor, Vps36, compromised exosome secretion but not the secretion of vesicles derived from nanotubes. The exosomes enter recipient trypanosome cells. Time-lapse microscopy demonstrated that cells secreting exosomes or purified intact exosomes affect social motility (SoMo). This study demonstrates that exosomes are delivered to trypanosome cells and can change their migration. Exosomes are used to transmit stress signals for communication between parasites.

【 授权许可】

CC BY